To investigate the breeding system of plant species, Amos Dafni provided different pollination treatments. Using these one can disentangle if a species is self-compatible or depending on outcross-pollination. Furthermore, one can get initial sights of inbreeding depression and if a plant species is pollen or resource limited. Emasculation means cutting the anthers (carrying the pollen grains). Bagging is to be done by mesh nets or perforated pollen proof pollination bags. Hand-pollination can be carried out by using paint brushes or tooth picks.